American Society of Hematology

Figure 1

$Figure 1. FACS analysis of hMASCs. Representative flow cytometry histograms of multipotent cell populations. Plots show isotype control IgG-staining profile (open histogram) versus specific antibody staining profile (dash histogram). All the tested cell lines displayed a similar immunophenotype, being on an average (1) the cell fraction highly expressing CD13, CD49b, or CD90: 97% (± 7%), 84% (± 14%), and 79% (± 20%), respectively, and (2) the cell fraction expressing low levels of CD73, CD44, HLA-ABC, CD29, CD105, KDR, or CD49a: 93% (± 10%), 91% (± 17%), 85% (± 6%), 79% (± 15%), 75% (± 16%), 72% (± 14%), and 70% (± 9%), respectively. The vast majority of the cell population (> 99%) was, instead, negative for CD14, CD45, CD38, HLA-DR, CD133, CD117, and CD34.$

FACS analysis of hMASCs. Representative flow cytometry histograms of multipotent cell populations. Plots show isotype control IgG-staining profile (open histogram) versus specific antibody staining profile (dash histogram). All the tested cell lines displayed a similar immunophenotype, being on an average (1) the cell fraction highly expressing CD13, CD49b, or CD90: 97% (± 7%), 84% (± 14%), and 79% (± 20%), respectively, and (2) the cell fraction expressing low levels of CD73, CD44, HLA-ABC, CD29, CD105, KDR, or CD49a: 93% (± 10%), 91% (± 17%), 85% (± 6%), 79% (± 15%), 75% (± 16%), 72% (± 14%), and 70% (± 9%), respectively. The vast majority of the cell population (> 99%) was, instead, negative for CD14, CD45, CD38, HLA-DR, CD133, CD117, and CD34.

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