NK cells with the highest surface density of Tim-3 secrete the most IFN-γafter IL-12 and IL-18 stimulation. (A) Plots depict coexpression of Tim-3 and IFN-γ production in unstimulated (top) or stimulated by IL-12 and IL-18 (bottom) NK-cell subsets (CD56^brightCD16^– [left plots] and CD56^dimCD16⁺ [right plots]) in a representative healthy donor. (B) Graph shows frequency of IFN-γ⁺ NK cells based on Tim-3 expression on NK-cell subsets (CD56^brightCD16^– [solid circles] and CD56^dimCD16^– [open circles]) after IL-12 and IL-18 stimulation in 6 unrelated healthy donors. (C) Histograms show Tim-3 surface expression on IFN-γ⁻ (gray shade) and IFNγ⁺ (black line) cells gated from IL-12– and IL-18–stimulated NK-cell subsets (CD56^brightCD16^– [left] and CD56^dimCD16⁺ [right]) from a representative donor (n = 6).