Gene silencing of MR reduces uptake of ADAMTS13 by iDCs. Silencing of MR in iDCs reduces uptake of ADAMTS13. iDCs were transfected with nontargeting siRNA or targeting MR and DC-SIGN siRNAs. After 72 hours, the expression of MR and DC-SIGN was measured by FACS (A,C). After siRNA transfection, cells were incubated with 50nM ADAMTS13-488 and its uptake was monitored by FACS (B,D). Dotted line histograms represent cells transfected with siRNAs targeting MR and DC-SIGN (KD). Solid line histograms represent cells transfected with nontargeting siRNAs (NT). Gray histograms represent cells stained with isotype control Abs. Graphs represent data from 3 independent experiments and are expressed as means ± SD. Results are expressed as a percentage of the mean fluorescence intensity, where 100% corresponds to the highest mean fluorescence intensity obtained with ADAMTS13-488.