CLRs mediate ADAMTS13 uptake by iDCs. FACS analysis of ADAMTS13-488 uptake by iDCs is shown. (A-B) Uptake of ADAMTS13 (expressed as a percentage) was prevented by preincubation of the cells with 5mM EGTA. (C) Inhibition of ADAMTS13-488 endocytosis by different monosaccharides. The gray histograms represent controls incubated in the absence of ADAMTS13. Cells incubated with ADAMTS13 are indicated by a solid line; cells incubated with ADAMTS13 and different sugar components are indicated by a dotted line. (D) Blocking of ADAMTS13 uptake by different sugars is expressed as a percentage. (E) Confocal analysis of the effect of mannan on ADAMTS13 endocytosis. iDCs were incubated with or without mannan before the addition of ADAMTS13-488 and stained with anti–DC-SIGN Ab (red).