Uptake of ADAMTS13 by iDCs is mediated by a clathrin-dependent cell surface receptor. Immature DCs were pre-incubated with 0.75M sucrose before the addition of 50nM ADAMTS13-488. (A) Cells were analyzed by FACS. Gray histogram represents control cells without ADAMTS13-488. (B) Data from 3 independent experiments ± SD are presented and are expressed as a percentage of mean fluorescence intensity, where 100% corresponds to the mean fluorescence signal obtained for ADAMTS13-488 in the absence of sucrose. Lucifer yellow (LY), which is internalized by macropinocytosis, was used as a control.