Phospho-p38 expression in PS KO T cells and in TK1 cells transfected with Dtx-1. Splenocytes from control or PS KO animals were harvested and labeled with FITC anti–phospho-p38 mAb in the presence of sodium orthovanadate (A). To check the effect of deltex-1 expression over p38 activation in thymocytes, TK1 cells were transfected with pMIGR-DELTEX1 vector containing this gene or empty vector. Than, stable TK1 cell lines were sorted by GFP expression and stimulated (or not) for 10 minutes with anti-CD3 before protein extraction (B). Results are representative of 3 animals (A); one experiment representative of three (B).