Cell death, T-cell turnover, and Vβ family expression in PS KO animals. Splenocytes from control or PS KO animals were harvested and stained with anti-CD4 APC, anti-CD8α PerCP, annexin-V PE, and 7-AAD (A), or FITC anti-BrdU mAb (B), or a panel of fluorescent-labeled mAbs against Vβ families (C). Only animals used to detect BrdU incorporation were intraperitoneally injected with BrdU (2 doses, 4 hours and 8 hours before splenocyte harvesting). Each bar represents mean and standard deviation of 3 animals. (A) Value in each quadrant represents the percentage of splenocytes labeled with annexin-V or 7-AAD.