FISH analysis of CYP3A. Hybridization of the CYP3A probe (labeled in green) and a chromosome 7 centromeric probe (labeled in red) in tumoral T cells. (A,B) Representative FISH images of MOLT16 cells. (C,D) FISH images of PEER cells. (E,F) FISH images from 2 paraffin-embedded PTCLs, showing cells with multiple CYP3A and centromeric 7 signals. The case PTCL-21 corresponds to panel E. (G) CYP3A4 mRNA content of 19 PTCL cases classified according to the number of CYP3A copies per cell, as assessed by FISH (CYP3A = 2 corresponds to 2 copies per cell; CYP3A > 2 corresponds to cases with more than 2 copies per cell). The mean CYP3A4 mRNA content for PTCL cases with 2 CYP3A copies and for those with more than 2 CYP3A copies is shown with horizontal dashed lines (0.9- and 11.6-fold change relative to lymph nodes, respectively; P < .001).