Intracellular IFN-γ production. (A) The graph shows the summary of intracellular IFNγ in p110γ- or p110δ-deficient NK cells in the absence of IL-2. (B) The graph shows the summary of intracellular IFNγ in p110γ- or p110δ-deficient NK cells in the presence of IL-2. Means and SDs are shown (n = 4-8). The ordinates indicate IFN-γ⁺ NK-cell population (%). *P = .016. (C-F) NK cells of the indicated genotypes were treated with DMSO, 10 μM LY294002, or 1 μM AS252424 and stimulated with anti-NK1.1 mAb for 5 hours in the presence of IL-2. The figures indicate the percentage of IFN-γ⁺ cells (measured against an isotype control) within gated NKG2D⁺CD3⁻ NK cells. One representative of 3 independent experiments for each genotype is shown.