GATA-1 and GATA-2 directly regulate many of the same genes during megakaryocytic development. (A) Mosaic plots showing that GATA-1–bound genes are significantly enriched for genes that are differentially expressed after restoration of GATA-1 in G1ME cells. (B) GATA-2–bound genes are significantly enriched for genes that are differentially expressed after shRNA-mediated down-regulation of GATA-2 in G1ME cells. (C) GATA-2–bound genes are significantly enriched for genes bound by GATA-1. The mosaic plots show the relative numbers of genes in each category as the area of the corresponding rectangle. If the whitespace between rows in each column are perfectly aligned, the response of the genes to the condition on the y-axis is independent of their categorization according to the condition on the x-axis. Deviations from perfect alignment represent enrichment or depletion as a condition of the y-axis category. The total number of genes in each category is shown inside the boxes. (D) Venn diagram showing the intersection of gene lists bound by GATA-1 and GATA-2. A total of 72% of GATA-1–occupied genes and 61% of GATA-2–occupied genes are bound by both factors. (E) Genes differentially expressed after shRNA-mediated down-regulation of GATA-2 are significantly enriched for genes differentially expressed after restoration of GATA-1 in G1ME cells. (F-H) GATA1 (top) and GATA2 (bottom) binding profiles at the Hhex, Mpl, and Epor loci. Peaks represent sequencing tag counts aligning to that genomic position.