Gene expression profiling of BL and DLBCL. (A) Molecular classification of pediatric lymphomas using the Bayesian compound covariate predictor method and a published gene signature that distinguishes BL from DLBCL.15 GEPs of adult lymphomas used to derive this signature were used as a training (GEO accession no. GSE4732) dataset for the Bayesian predictor. Pediatric lymphomas with a probability ≥ 90% were classified accordingly. (B) Hierarchical clustering of adult (n = 21) and pediatric (n = 49) BL and adult (n = 102) and pediatric (n = 21) DLBCL cases using the gene expression signature used in panel A demonstrated robust distinction of BL and DLBCL regardless of patient age. Pediatric and adult specimens intermingled and did not form distinct subclusters. (C) Hierarchical clustering of BL and DLBCL using 35 significantly differentially expressed miRNAs between BL and DLBCL. Cases clustered by entity regardless of patient age. Two adult DLBCL cases clustered with BL and one pediatric BL case clustered with DLBCL. A separate cluster of DLBCL (far right) demonstrated high expression of both the BL and DLBCL signatures.
