Modified lipid metabolism in PPARγ-instructed DCs. (A) Nile red uptake of control or PPARγ ligand (RSG)–treated DCs (5 days). Cells were stained with Nile red as described in “Nile red staining” for detection of lipid droplets. (B) Osmium tetroxide staining of DCs. DCs were cultured for 24 hours and control or RSG-treated cells were stained with osmium tetroxide. The arrows indicate the intracellular lipid droplets. See “Lipid body staining” for image acquisition information.