Gene-expression changes during DC development and upon PPARγ ligand treatment. (A) Probe sets (14 723) were regulated upon DC differentiation. Gene-expression data were normalized to freshly isolated monocytes. Dendograms were obtained from hierarchical clustering (standard correlation). Data were obtained using 6 biologic replicates. Cells were treated with 1 μM rosiglitazone (RSG; 6 hours, 24 hours DC) or with 2.5 μM RSG (5 days DC). RNA was isolated at the indicated time points. (B) Probe sets (1166) were regulated by PPARγ ligand during monocyte-derived DC differentiation. Microarray data were normalized to vehicle-treated cells. Venn diagram of the probe sets that were up-regulated (C) or down-regulated (D) after 5 days of PPARγ ligand treatment. Comparison of genes that were regulated in 5-day DCs versus monocytes is shown.