Lnk co-localizes with MPLWT and MPLW515L in the plasma membrane. (A) BaF/3 cells were cotransfected with either GFP-MPLWT or GFP-MPLW515L and V5-tagged Lnk and grown in the presence of Tpo (1 ng/mL). Cells were stained for MPL (MPL antibody, red) and Lnk (V5 antibody, blue). Transfected cells were detected by GFP fluorescence (green). The merged images show co-localization of MPL and Lnk (pink). (B) 293T cells were transfected with V5-wild type Lnk (LNKWT), V5-Lnk R392E (R392E), V5-Δ SH2 (aa 1-307), or V5-Δ PH-SH2 (aa 1-205) and stained with a V5 antibody (green). Stained cells were examined by confocal microscopy. (C) Schematic presentation of Lnk expression vectors.