Verification of HIV-1 infection of HPC-CFUs by Alu-LTR integration. (A) Infected colonies and blank wells were tested for integration using nested PCR.17 The resulting DNA product was approximately 150 bp in length. 8E5 cellular DNA (lanes 1-4: 50 000, 5000, 500, and 50 copies, respectively), a blank MJ4 well as the negative control (lane 5), MJ4-infected BFU-E colonies (lanes 6-8), MJ4-infected CFU-GM colonies (lanes 9-13), the MHX-infected CFU-GM colony (lane 14), BW-2036–infected BFU-E colonies (lanes 15, 16), BW-2036–infected CFU-GM colonies (lanes 17, 18), and H2O (lane 19) are shown. (B) Representative fluorescent curves of SYBR Green integration assay. Standards are indicated as copy number of 8E5 cellular DNA; positive colony types and negative blank wells from MJ4 and patient 13 are also shown with colors indicated in parentheses.
