Demethylating agents upregulate expression of CD44 in neoplastic MCs. (A-B) HMC-1, ROSA, and MCPV-1.1 cells were incubated with decitabine (0.1-5 μM) (A) or 5-azacitidine (0.1-5 μM) (B) at 37°C for 96 hours. Then, expression of CD44 was analyzed by flow cytometry. Bars represent the expression of CD44 (SI) as percentage of control (Co) expressed as mean ± SD of 3 independent experiments. *P < .05 compared with control (Student t test).