RUNX1 binds to and positively regulates genes involved in cell adhesion, cell migration, and cell-ECM interaction in the HE. (A) (Left) Venn diagram showing the overlap between RUNX1-bound and differentially expressed genes in HE. (Right) Heat map depiction of the 235 RUNX1-bound and differentially expressed genes. RUNX1 positively correlated genes are marked in red shades and negatively correlated genes are marked in blue shades. The genes are ordered according to fold change in differential expression (from high to low). Red boxes show components of the integrin signaling pathway. (B) Enriched signaling pathways in RUNX1-bound positively correlated genes as determined by IPA. (C) Depiction of integrin signaling pathway with RUNX1-bound positively correlated targets shown in red. Integrins mediate signaling through the focal adhesion kinase and SRC-family kinases to mediate cytoskeletal rearrangements by either activating the Arp2/3 complex through the Wiskott–Aldrich syndrome protein, or by activating the myosin light (MYL9 or MLC) chain through phosphorylation from the MYL9 kinase (MYLK or MLCK). (D) qPCR analysis on iRunx1^Runx1−/− HE cells at 0 and 6 hours postdox treatment. Graphs show fold change relative to no dox treatment of 3 biological replicates. *P < .05. Paired Student t test was used for statistical analysis.