CUX1 knockdown leads to a partial block in erythropoiesis. Flow cytometric analysis of BM and spleen erythroblast populations was performed for: (A-B) Cux1^mid mice treated with dox for 18 months; (C-D) Cux1^low mice at the time of euthanization for disease; (E) primary BMT recipients 3 months after transplantation; and (F) primary BMT recipients at the time of euthanization. (G) Absolute count of all RI through RIV erythroblasts in the BM and spleens of BMT recipients at the time of euthanization is provided. Age-matched, littermate control Ren mice were used for all experiments. The erythroid cell populations from least to most differentiated are indicated as RI through RIV. RI, pro-erythroblasts; RII, basophilic erythroblasts; RIII, polychromatophilic erythroblasts; RIV, orthochromatophilic erythroblasts. (A-D) Representative flow analysis. The mean ± SD is shown. All data were analyzed with Wilcoxon rank test, *P ≤ .05, **P ≤ .01, ***P ≤ .001.