A state of adapted homeostasis is experienced after resolving inflammation. PKH26-PCL^red was injected into the cavity of naïve WT and ccr2^−/− mice followed 2 hours later by 0.1 mg of zymosan. Six days after zymosan PKH26-PCL^green was injected to distinguish resMφs (PKH26-PCL^gred and PKH26-PCL^green) from infiltrating moMφs/DCs (PKH26-PCL^green only). The peritoneal cavity of these mice was examined 60 days after the initial zymosan injection revealing (A) a population of moMφs that were PKH26-PCL^green, but were absent in ccr2^−/− mice alongside (B) moDC numbers and (C) T-cell activation markers. (D) The resMφs and moDCs were FASCsorted for phenotypic analysis, whereas (E) the impact of postresolution altered homeostasis to a second hit of S pneumonia was determined on day 60. The P value was ≤.05, as determined by ANOVA, followed by the Bonferroni t test or two-tailed Student t test, with data expressed as mean ± SEM for n = 6 mice/group. *P ≤ .05; **P < .01; ***P < .001.