Figure 3
Aspects of resolution-phase Mφ phenotype are conserved between sterile and infections resolving inflammation. (A) The temporal profile in resMφs, moMφs, and DCs are shown throughout the inflammatory and postresolution response to S pneumoniae. These cells, as well as the equivalent population from 0.1 mg of zymosan, were shown (B-C) FACSorted and subjected to reverse transcription polymerase chain reaction. Injecting CFSE-labeled apoptotic PMNs into the peritoneum 48 hours post 0.1 mg of zymosan confirmed that (D) resMφs preferentially phagocytosed apoptotic PMNs with a little role for moMφs in this process; data confirmed using (E) Ly6chi-deficinet ccr2−/− mice showing no buildup of PMNs in the cavity postresolution. Data are presented as mean ± SEM for n = 6 mice/group. i.p., intraperitoneal.

Aspects of resolution-phase Mφ phenotype are conserved between sterile and infections resolving inflammation. (A) The temporal profile in resMφs, moMφs, and DCs are shown throughout the inflammatory and postresolution response to S pneumoniae. These cells, as well as the equivalent population from 0.1 mg of zymosan, were shown (B-C) FACSorted and subjected to reverse transcription polymerase chain reaction. Injecting CFSE-labeled apoptotic PMNs into the peritoneum 48 hours post 0.1 mg of zymosan confirmed that (D) resMφs preferentially phagocytosed apoptotic PMNs with a little role for moMφs in this process; data confirmed using (E) Ly6chi-deficinet ccr2−/− mice showing no buildup of PMNs in the cavity postresolution. Data are presented as mean ± SEM for n = 6 mice/group. i.p., intraperitoneal.

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