Effect of integrilin on distribution of prothrombinase constituents. (A) Kinetics of Alexa₄₈₈-FV-810^SYA (600 µg/kg), Alexa₆₄₇-FXa_i (80 µg/kg), and (B) platelet fluorescence accumulation over time in the presence (n = 5 mice; 23 thrombi) or absence (n = 5 mice; 22 thrombi) of integrilin. (C) Confocal imaging of labeled platelets, Alexa₄₈₈-FV-810^SYA, and Alexa₆₄₇-FXa_i 240 seconds after laser injury, merged or presented individually; step size = 1 µm in z-plane; total section 30 µm. (C) Without integrilin (top); with integrilin (bottom). Platelet accumulation was monitored using Alexa₅₅₅-labeled rat anti-CD41 F_(ab)2 antibody.