Expression of mVWF in VWF^−/− mice after hydrodynamic injection. (A) VWF:Ag levels after hydrodynamic injection (group data). VWF^−/− mice were sampled every 7 days after hydrodynamic injection of the VWF expression plasmid (n > 8 for each group). (B) VWF:Ag levels after hydrodynamic injection (individual mouse responses). VWF knockout mice expressing collagen-binding mutants were sampled after hydrodynamic injection (n > 8). The levels of VWF:Ag for each individual mouse are shown at 14, 21, 28, and 36 days following the hydrodynamic delivery. The horizontal lines indicate mean values for each group.