CD3 and TCRαβ expression on the surface of T-cell subsets. (A) Expression of CD3 (red line) or isotype (solid gray) on the surface of CD4⁺ FOXP3⁻ T_conv, CD4⁺FOXP3⁺ T_reg, and CD8⁺ T cells. (B) Cumulative data of geometric mean for mean fluorescence intensity of CD3 surface expression among T_conv, T_reg, or CD8⁺ cells. (C) Expression of TCRαβ (red line) or isotype (solid gray) on the surface of T_conv, T_reg. and CD8⁺cells. (D) Cumulative data of geometric mean for mean fluorescence intensity of TCRαβ surface expression among T_conv, T_reg, and CD8⁺ cells. (B,D) Five patients (P1-P5) were studied.