Molecular transport is increased in diYF thrombi. cAlb was used to calculate molecular transport in thrombi formed following laser-induced injury in cremaster arterioles of WT and diYF mice. (A) Photomicrographs show caged-carboxyfluorescein albumin fluorescence within a representative WT and diYF thrombus. The fluorescence is shown in pseudocolor to indicate relative concentration (blue is low, red is high). From left to right, panels show the same WT (top row) or diYF (bottom row) thrombus immediately following a flash of 405-nm laser light to uncage the cAlb in the thrombus (0 seconds), followed by images captured 1 second and 2 seconds after the flash. The decrease in albumin fluorescence from 0 to 2 seconds is due to washout of the albumin from the platelet mass. Note the albumin is retained longer in the core region of the thrombi. (B) Graph shows cAlb washout in the core (P-selectin positive, triangles) and shell (P-selectin negative, squares) regions of WT (blue) and diYF thrombi (red). Quantification and normalization procedure is described in “Methods.” Values are mean ± SEM for n = 11 WT and n = 12 diYF thrombi.