Amounts of tumor-produced BART miRNAs in EBV⁺DLBCL in elderly patients. (A) EBV-encoded noncoding RNA (EBER) served as a highly sensitive marker to detect EBV infection. EBER and BART13 were detected from DLBCL biopsy samples by fluorescence in situ hybridization. Representative results are shown. The definition of group 1 (n = 6) and 2 (n = 7) is indicated in B. (B) Comparison of the index of BART13⁺ area/number of EBER⁺ cells. Group 1, BART/EBER <10; gGroup 2, BART/EBER >10. *P < .05. (C-D) The numbers of EBER⁺ cells and BART13⁺ cell areas were automatically counted. Comparison of the number of EBER⁺ cells (C) and BART13⁺ cell areas (D) in the tissue area on a slide. *P < .05. (E) Survival ratio of patients with DLBCL. *P = .0004, log-rank test. (F) BART miRNAs were detected from EBV⁺ DLBCL (top) and EBV⁺ Hodgkin’s lymphoma (bottom) biopsy samples by in situ hybridization. (Left) Tumor cells; (middle) macrophages; (right) negative staining (using scramble miRNAs for the probe). (Top) BART-13 was detected; (bottom) BART-2. Representative BART miRNA signals (blue dots) are indicated by arrows. Scale bar, 10 μm.