Effect of ST3GAL6 knockdown on tumor burden and survival in mice. (A) Tumor burden as assessed by BLI: shown are weeks 1, 2, and 3, and final imaging at week 4 post-injection of 5 × 106 cells (n = 6/group). A reduction in tumor burden was noted in mice that received shST3GAL6 cells compared with scrambled controls. (B) Average BLI signal was quantified over the course of the 4-week study. This demonstrated a statistically significant reduction in tumor burden for mice that received shST3GAL6 cells compared with scrambled controls cells; P = .005. (C) Kaplan-Meier survival probability curve. The effect of knockdown of ST3GAL6 in MM cells on survival of xenograft mice was evaluated in a separate survival study. Mice injected IV with 5 × 106 shST3GAL6 cells had a superior survival to mice injected with scrambled control cells (n = 7/group), log-rank P = .0014.
