Figure 6.
Figure 6. Absence of selenoproteins in macrophages and monocytes delays stress erythropoiesis. (A-B) Flow cytometry analysis of F4/80+ macrophages in the spleens of Se-A and Se-D mice treated with PHZ. (A) 100% PHZ treatment. Percentage F4/80+ cells were measured on the indicated days; n = 3 per diet. (B) 50% PHZ treatment. Percentage (left) and total numbers (right) of F4/80+ cells on indicated days after 50% PHZ treatment; n = 4 per diet. (C) Analysis of mice with deletion of Trsp in myeloid cells. Trspfl/flLysMCre mice were treated with 100% PHZ and hematocrit levels were measured on the indicated days after treatment; n = 3-4 per group. (D-G) Analysis of macrophage and monocyte populations in the spleens of Se-A and Se-D mice treated with 50% PHZ. (D-E) Flow cytometry analysis of (D) F4/80+CD11blo/− cells and (E) F4/80+CD11b+ cells; n = 4 per diet. (F) Flow cytometry analysis of spleen RPMs (F4/80+CD11blo/−Vcam1+) after 50% PHZ treatment. Percentage of RPMs (left) and total numbers (right); n = 4 per diet. (G) Flow cytometry analysis of CD11b+CD115+ monocytes in the spleen on the indicated days after 50% PHZ treatment. Percentage (left) and total numbers (right) of monocytes; n = 4 per diet. (H-K) Quantitation of monocyte and macrophage populations in the spleen of TrspΔ/Δ or control cells transplanted mice on days 8 and 10 after BMT; n = 4 per group. (H) F4/80+CD11blo/− cells, (I) F4/80+CD11b+ cells, (J) RPMs, (K) monocytes. (L) Analysis of Spic expression in the spleen on the indicated days after PHZ treatment by quantitative reverse transcription polymerase chain reaction. 100% PHZ (left; n = 3-7 per diet) and 50% PHZ (right; n = 3-4 per diet). (M) Rescue of Se-D defects in spleen macrophages by adoptive transfer of monocytes. CD11b+Ly6G− monocytes were isolated from spleens of Se-A mice by magnetic bead enrichment and injected into Se-D mice 6 hours after 50% PHZ treatment. Hematocrit values were measured as indicated; n = 3 for each group. Bars are representative of mean ± SEM. *P < .05; **P < .001; ***P < .005; ****P < .0001.

Absence of selenoproteins in macrophages and monocytes delays stress erythropoiesis. (A-B) Flow cytometry analysis of F4/80+ macrophages in the spleens of Se-A and Se-D mice treated with PHZ. (A) 100% PHZ treatment. Percentage F4/80+ cells were measured on the indicated days; n = 3 per diet. (B) 50% PHZ treatment. Percentage (left) and total numbers (right) of F4/80+ cells on indicated days after 50% PHZ treatment; n = 4 per diet. (C) Analysis of mice with deletion of Trsp in myeloid cells. Trspfl/flLysMCre mice were treated with 100% PHZ and hematocrit levels were measured on the indicated days after treatment; n = 3-4 per group. (D-G) Analysis of macrophage and monocyte populations in the spleens of Se-A and Se-D mice treated with 50% PHZ. (D-E) Flow cytometry analysis of (D) F4/80+CD11blo/− cells and (E) F4/80+CD11b+ cells; n = 4 per diet. (F) Flow cytometry analysis of spleen RPMs (F4/80+CD11blo/−Vcam1+) after 50% PHZ treatment. Percentage of RPMs (left) and total numbers (right); n = 4 per diet. (G) Flow cytometry analysis of CD11b+CD115+ monocytes in the spleen on the indicated days after 50% PHZ treatment. Percentage (left) and total numbers (right) of monocytes; n = 4 per diet. (H-K) Quantitation of monocyte and macrophage populations in the spleen of TrspΔ/Δ or control cells transplanted mice on days 8 and 10 after BMT; n = 4 per group. (H) F4/80+CD11blo/− cells, (I) F4/80+CD11b+ cells, (J) RPMs, (K) monocytes. (L) Analysis of Spic expression in the spleen on the indicated days after PHZ treatment by quantitative reverse transcription polymerase chain reaction. 100% PHZ (left; n = 3-7 per diet) and 50% PHZ (right; n = 3-4 per diet). (M) Rescue of Se-D defects in spleen macrophages by adoptive transfer of monocytes. CD11b+Ly6G− monocytes were isolated from spleens of Se-A mice by magnetic bead enrichment and injected into Se-D mice 6 hours after 50% PHZ treatment. Hematocrit values were measured as indicated; n = 3 for each group. Bars are representative of mean ± SEM. *P < .05; **P < .001; ***P < .005; ****P < .0001.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal