Figure 4.
Figure 4. Transcriptomic analysis of ProEs during stress erythropoiesis. (A-B) Affymetrix transcriptomic analysis of sorted splenic ProEs from Se-D or Se-A mice on day 3 after 50% PHZ treatment; n = 3 per diet. (A) Heat map of gene hierarchical clustering analysis with cutoff at |fold change|> 2. (B) Scatter plot of gene differential expression analysis with cutoff at |fold change|> 2. (C) Pathway enrichment analysis. Pathways of interest with P < .05 are shown. (D) Heat map representation of specific gene expression differences. (E) Quantitative reverse transcription polymerase chain reaction analysis of specific genes to confirm differential expression. RNA was isolated from ProEs sorted from Se-D and Se-A mice on day 3 after 50% PHZ treatment. (F) Western blot analysis (top) and densitometry (bottom) of Bach1 in sorted splenic ProEs from mice on day 3 after 50% PHZ treatment; n = 2-3 per diet. Bars are representative of mean ± SEM.

Transcriptomic analysis of ProEs during stress erythropoiesis. (A-B) Affymetrix transcriptomic analysis of sorted splenic ProEs from Se-D or Se-A mice on day 3 after 50% PHZ treatment; n = 3 per diet. (A) Heat map of gene hierarchical clustering analysis with cutoff at |fold change|> 2. (B) Scatter plot of gene differential expression analysis with cutoff at |fold change|> 2. (C) Pathway enrichment analysis. Pathways of interest with P < .05 are shown. (D) Heat map representation of specific gene expression differences. (E) Quantitative reverse transcription polymerase chain reaction analysis of specific genes to confirm differential expression. RNA was isolated from ProEs sorted from Se-D and Se-A mice on day 3 after 50% PHZ treatment. (F) Western blot analysis (top) and densitometry (bottom) of Bach1 in sorted splenic ProEs from mice on day 3 after 50% PHZ treatment; n = 2-3 per diet. Bars are representative of mean ± SEM.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal