Se deficiency impairs erythroblast maturation. (A-B) Flow cytometry analysis and representative graphs of splenic erythroblasts after 50% PHZ treatment. (A) ProEs and (B) BasoEs are characterized by CD71^hiTer119^int and CD71^hiTer119^hiFSC^hi, respectively. (C) Percentage BasoEs (left) and total numbers of BasoEs (right) isolated from the spleens of Se-A and Se-D mice on the indicated days after 50% PHZ treatment; n = 4 per diet. (D) Percentage ProEs (left) and total numbers of ProEs (right) isolated from the spleens of Se-A and Se-D mice on the indicated days after 50% PHZ treatment; n = 4 per diet. (E-H) Characterization of erythroblasts from Trsp^Δ/Δ or control cells transplanted mice on days 8 and 10 after BMT. (E) Flow cytometry analysis of total erythroblasts and (F) Ter119^hi cells. (G) Quantitation of spleen ProEs and (H) BasoEs. (I) Western blot analysis (left) and densitometry (right) of GATA-1 in sorted splenic ProEs from mice on day 3 after 50% PHZ treatment; n = 3 per diet. Bars are representative of mean ± SEM. Panels B and F were gated on Ter119hi cells. *P < .05; **P < .001; ***P < .005; ****P < .0001.