Se deficiency impairs stress erythropoiesis after PHZ administration. (A) Schematic of the study model. C57BL/6 mice were fed on a Se-D or Se-A diet for 12 weeks before use. Then mice were treated with PHZ (100 mg/kg [100%] or 50 mg/kg [50%]). Kinetics of stress erythropoiesis were evaluated by time course study. (B-G) Kinetics of stress erythropoietic recovery of Se-D and Se-A mice treated with 100% PHZ. (B) Survival curve of mice over the course of 12 days of recovery; n = 8 per diet. (C) Hematocrit measurements of mice during the recovery from PHZ-induced anemia; n = 10-15 per diet. (D) Normalized reticulocyte percentage in the whole blood at days 1.5 and 2.5 after PHZ treatment; n = 3 per diet. (E) Spleen weights (left) and the representative pictures of spleens (right) from mice on the different diets on the indicated days after PHZ treatment; n = 3-7 per diet. (F) Hematoxylin and eosin staining of spleen sections from Se-D and Se-A mice on the indicated days after PHZ treatment (original magnification ×100). (G) Stress BFU-E colony assays of spleen cells isolated from Se-D and Se-A mice at indicated times after PHZ treatment (left) and representative pictures of BFU-E colony morphology on day 3 posttreatment (right; acid benzidine stain; original magnification ×50); n = 3-6 per diet. Bars are representative of mean ± SEM. **P < .001; ***P < .005; ****P < .0001.