BCR-mediated NFATc1 activation regulates IL-10 expression in DLBCL cells. (A) OCI-LY10 and HBL-1 cells were treated with different BTK inhibitors (ibrutinib, ACP-196, and BGB-3111) at different doses for 24 hours. Supernatants collected from these samples were used to analyze IL-10 secretion using ELISA. (B) OCI-LY10 and HBL-1 cells were transfected with validated NFATc1 or control shRNA plasmids. After a 72-hour incubation, ELISA was used to examine IL-10 secretion in the supernatants, and purified nuclear extracts from the transfected cells were analyzed for NFATc1, pSTAT3, and lamin B (loading control) expression by western blotting. (C) Bar graphs show results of qPCR of regions (amplicon 1) pulled down by the NFATc1 antibody as fold enrichment relative to the background signals from the isotype-control immunoglobulin G antibody. *P < .05. NS, not statistically significant.