Flow cytometry and transmission electron microscopy assessment of platelet function in patients III:3 and III:5. Flow cytometry assessment of platelet function in patients III:3 and III:5 assessed on an Accuri C6 flow cytometer. (A) Platelet glycoprotein receptors. (B-C) CD62P expression (B) and fluorescent fibrinogen binding (C) following platelet stimulation by various agonists for 2 minutes. The PRP from healthy controls was diluted 1:10 with phosphate-buffered saline and served as a control range. Data for healthy volunteers are shown as mean ± 1 SD (n = 9; except for GPVI, where n = 2). (D) Transmission electron microscopy image of the platelets from patient III:5 and healthy control platelets. Arrow indicates an α-granule, and the graph shows the number of α-granules per surface area. Scale bar, 2 µm. ADP, adenosine 5′-diphosphate.