Reduced erythropoietic supporting ability of Ezh2-deficient endothelium. (A) Gating strategy used to define stromal cell (SC; Ter119⁻CD45⁻CD31⁻) and endothelial cell (EC; Ter119⁻CD45⁻CD31⁺Lyve1⁺) populations within the total FL live (7AAD⁻) singlet population. Proportion (B) and absolute numbers (C) of SCs and ECs (WT, n = 8; KO, n = 7; 2 independent experiments) per FL at E12.5. (D) Experimental design of the coculture assay. (E) Measurement of WT or Tie2-Ezh2-KO E12.5 FL SC erythropoietic supporting ability with coculture assay (2 independent experiments). LSK indicates LSK cells only. SC indicates SCs only. (F) Measurement of WT, Tie2-Ezh2-KO, or mKitL-KO E12.5 FL EC (Ter119⁻CD45⁻CD31⁺) erythropoietic supporting ability with coculture assay (7 independent experiments). LSK indicates LSK cells only. EC indicates ECs only. (G) Measurement of WT or Tie2-Ezh2-KO E12.5 FL EC myeloid supporting ability with coculture assay (2 independent experiments). Numbers of replicates are indicated at the bottom of each column. Two-tailed Student t tests were used to assess statistical significance. Error bars represent SEM.