American Society of Hematology

Figure 1.

$Figure 1. A novel fusion gene of AGGF1-PDGFRB in Ph-like ALL. (A) Schematic representation of the treatment course of the index patient with the AGGF1-PDGFRB fusion gene. Samples at diagnosis, at the end of induction (chemotherapy refractory), and at relapse were chosen for WGS and RNA-seq. (B) Schematic diagrams of AGGF1-PDGFRB based on the RNA-seq and WGS analyses. (C) Growth rate of Ba/F3 cells with AGGF1-PDGFRB, EBF1-PDGFRB, or empty lentivirus vector (mock) after removal of interleukin 3 (IL-3). (D) Localization of AGGF1-PDGFRB and EBF1-PDGFRB in Ba/F3 cells was detected by immunofluorescence (donkey anti-rabbit Alexa Fluor plus 555 and DAPI stain; original magnification ×200). (E) Lysate of Ba/F3 cells with AGGF1-PDGFRB, EBF1-PDGFRB, BCR-ABL1, and empty vector (mock) with or without IL-3 (10 ng/mL) to detect the phosphorylation of Stat5, Akt, and Erk1/2. All experiments were performed in triplicate. BM, bone marrow; DAPI, 4′,6-diamidino-2-phenylindole; pErk, phosphorylated Erk; WT, wild type.$

A novel fusion gene of AGGF1-PDGFRB in Ph-like ALL. (A) Schematic representation of the treatment course of the index patient with the AGGF1-PDGFRB fusion gene. Samples at diagnosis, at the end of induction (chemotherapy refractory), and at relapse were chosen for WGS and RNA-seq. (B) Schematic diagrams of AGGF1-PDGFRB based on the RNA-seq and WGS analyses. (C) Growth rate of Ba/F3 cells with AGGF1-PDGFRB, EBF1-PDGFRB, or empty lentivirus vector (mock) after removal of interleukin 3 (IL-3). (D) Localization of AGGF1-PDGFRB and EBF1-PDGFRB in Ba/F3 cells was detected by immunofluorescence (donkey anti-rabbit Alexa Fluor plus 555 and DAPI stain; original magnification ×200). (E) Lysate of Ba/F3 cells with AGGF1-PDGFRB, EBF1-PDGFRB, BCR-ABL1, and empty vector (mock) with or without IL-3 (10 ng/mL) to detect the phosphorylation of Stat5, Akt, and Erk1/2. All experiments were performed in triplicate. BM, bone marrow; DAPI, 4′,6-diamidino-2-phenylindole; pErk, phosphorylated Erk; WT, wild type.

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