Wt1-haploinsufficient hematopoietic cells partially progress to leukemic transformation over time. (A) Detail of samples submitted to detection of somatic mutations in selected published leukemia driver genes: M-IMPACT (left panel). Detail of mutations, colored by mutation type, found in secondary and tertiary transplanted diseased mice (right panel). (B) Kaplan-Meier survival curves of primary Wt1^fl/+ and control littermates (n = 7-14). Liver weights (C) and proportion of CD45⁺ cells and mature cells among these CD45⁺ cells (D) from livers of control and diseased Wt1^fl/+ mice (n = 4). (E) Histology of liver, BM, and SPL sections of aged primary heterozygous depleted mice (hematoxylin and eosin stain). (F) Quantification of mature and stem cells in the BM of control and diseased mice (n = 4). SPL weights (G) and quantification of mature cells (H) in the SPL of control and diseased mice (n = 4). Data are mean ± standard error of the mean (C-D,F-H). *P < .05, ***P < .001, ****P < .0001, Mantel-Cox test (B) or 2-way analysis of variance (C-D,F-H).