Wt1 depletion alters myeloid differentiation but does not markedly impact steady-state hematopoiesis. (A) The proportion of Lin⁻ cells and progenitors (common myeloid progenitors [CMPs], GMPs, and megakaryocyte/erythrocyte progenitors [MEPs]) were measured in the BM compartment of primary mice 1 month after Wt1 depletion (n = 5). (B) Methylcellulose colony-forming assay of sorted LSK cells from Wt1^fl/+ and Wt1^fl/fl mice and their control littermates. Representative results of 3 independent experiments, each performed in triplicate. Kaplan-Meier survival curves of secondary (C) and tertiary (D) recipients transplanted with control, Wt1^fl/+ or Wt1^fl/fl total BM cells (n = 10). (E) Thymus and lymph node weights of mice with T-ALL compared with control littermates (n = 3-5). (F) CD3⁺ cells fraction in the SPL, BM, and peripheral blood (PB) compartments of diseased mice compared with controls (n = 3-5). (G) Thymic percentages of CD4⁺, CD8⁺, and CD4⁺CD8⁺ cells among CD3⁺ cells of T-ALL and control mice at disease onset (n = 3-5). (H) Quantification of mature and c-Kit⁺ cells (%) in the peripheral blood and BM of control and diseased Wt1^fl/+ secondary recipients (n = 3-5). (I) Stem/progenitor cell percentages in the BM of T-ALL animals compared with controls (n = 3-5). Graphs show mean ± standard error of the mean. *P < .05, **P < .01, ****P < .0001, 2-way analysis of variance (A-B,E-I) or Mantel-Cox test (C-D). GEMM, granulocyte-erythrocyte-monocyte-megakaryocyte.