G6b-B–Shp2 regulates CLEC-2 signaling. Washed platelets (4 × 10⁸/mL) from G6b KO (A), G6b-B diY/F (B), Shp1 KO (C), and Shp2 KO (D) mice and litter-matched WT controls were activated with 10 μg/mL activating CLEC-2 antibody in the presence of 10 μM lotrafiban, followed by lysing and investigation of named phospho-tyrosine sites by western blotting. Representative western blots from 3 independent experiments. *P < .05; **P < .01.