Reduced platelet adhesion and activation under flow conditions. PPACK, heparin, and fragmin anticoagulated whole blood from WT, G6b-B diY/F and G6b KO mice was flowed for 3.5 minutes (1000 s⁻¹ shear) over coverslips coated with collagen, vWF-BP + laminin, and vWF-BP + laminin + rhodocytin. Representative brightfield images (A) and representative fluorescence images (B) following staining with Alexa Fluor 647–conjugated annexin V, P-selectin–FITC, and JON/A-PE antibodies to measure phosphatidylserine-positive platelets, α-granule release, and αIIbβ3 integrin activation (αIIbβ3^act), respectively (scale bars, 10 μm). (C) Heat map showing the effect size of morphology scores and surface area coverage (SAC) of the indicated parameters, normalized to WT (n = 5 or 6).