Aberrant platelet function in G6b-B diY/F mice. (A) Mean aggregation and ATP release traces in washed platelets (2 × 10⁸/mL). For ADP aggregations, platelets were washed in the presence of 0.02 U/mL apyrase and supplemented with 1 μM CaCl₂ and 50 μg/mL fibrinogen (mean ± SEM, n = 4 or 5). (Bi) Representative images of WT and G6b-B diY/F washed platelet (2 × 10⁷/mL) spreading on fibrinogen under basal conditions and 0.1 U/mL thrombin–preactivated conditions (scale bars, 5 μm). Quantification of surface area coverage (Bii), platelet perimeter (Biii), total platelets per image (Biv), and 4 stages of spreading in the presence of thrombin (Bv) (mean ± SEM, n = 5 or 6). ***P < .001.