Dysregulation of CBS7/9 boundary perturbs myeloid oncogenic transcription programs. (A) Heat map of RNA-seq analysis shows upregulated and downregulated genes upon heterozygous deletion of CBS7/9 in MOLM13 cells. (B) The CBS7/9 boundary–regulated genes were analyzed and annotated by Gene Ontology analysis. (C) qRT-PCR analysis and confirmation of representative downregulated genes identified in the RNA-seq analysis. (D) Heat map analysis of the affected pathways critical for HS/PC function upon heterozygous deletion of CBS7/9 in MOLM13 cells. (E) Enrichment of downregulated target genes involved in the MAPK signaling pathway in CBS7/9^+/− cells compared with WT control, as shown by GSEA. (F) Enrichment of upregulated target genes involved in the pathway important for myeloid and leukocyte migration and differentiation in CBS7/9^+/− cells compared with WT control, as shown by GSEA. (G) Overlap between downregulated (left) and upregulated (right) genes by RNA-seq analysis, and global decreases (left) and increases (right) in promoter chromatin accessibility by ATAC-seq analysis.