Structural elucidation at the FA level of lipids in the main platelet lipid categories. (A-D) Molecular structural analysis of lipids by shotgun lipidomics at the precursor and fragment level. The different panels showing different MS2 spectra that are used for structural assessment by customized mfql search files, using the fully automated search engine LipidXplorer. Potential fragmentation mechanisms are shown above each panel. Annotated fragments are denoted in the chemical structure of individual species. (E-I) Radar plots displaying the relative intensity of lipid species in resting platelets. The lipid species are presented according to their specific categories, including phospholipids (PL), GL, SL, FA, and derivatives (LM), and cholesterol and derivatives (Chol). PLs and GLs were analyzed by shotgun lipidomics and Chol, SL, and LM were analyzed by targeted lipidomics. All species were analyzed at least in 4 different independent biological experiments. DAG, diacylglycerol; TAG, triacylglycerol.