Abnormal dysplastic hematopoiesis in Srsf2 mutant-transplanted mice. (A) Complete blood cell (CBC) counts of the PB from WT and KI mice are plotted as dots. The mean ± SD are presented as bars. Data were obtained from 4 independent experiments. The numbers of mice analyzed were n = 45 (WT) and n = 41 (KI) at 8 weeks and n = 38 (WT) and 31 (KI) at 16 weeks after transplantation. (B) Kaplan-Meier survival curve of WT (n = 26) and KI (n = 23) mice at 300 days after transplantation. (C) Percentages of dysplastic erythroid cells, myeloid cells, and megakaryocytes are plotted; bars indicate mean ± SD. In total, 7 pairs of WT and KI mice were analyzed for morphological evaluation of erythroid and myeloid cells, and 5 pairs were analyzed for morphological evaluation of megakaryocytes. For each mouse, >200 erythroid cells, >100 myeloid cells, and >15 megakaryocytes were counted. (D) Representative May–Grünwald–Giemsa staining of BM cells is shown; arrows point to binucleated erythroid cells. Magnification 1000×; scale bar, 10 μm. (E) Upper panels: representative flow cytometry analysis of erythroid lineage in the spleen, showing CD71^highTer119^med (R1), CD71^highTer119^high (R2), CD71^medTer119^high (R3), and CD71^lowTer119^high (R4) gates. Lower panels: percentage of erythroid precursors in the BM and spleen cells (mean ± SD, n = 7). *P < .05; **P < .01; ***P < .001; ****P < .0001. BMT, bone marrow transplantation. KI, Srsf2 mutant transplanted mice; WT, wild-type transplanted mice.