HSP110 expression has an impact on ABC-DLBCL cell survival. (A) Kinetics of cell survival determined by annexin-V/7-aminoactinomycin D (7AAD) staining on shRNA-expressing cells, data are presented as the mean ± standard deviation (n = 3). (B) Representative annexin-V/7AAD staining on shRNA-expressing cells determined at day 9. (C) Immunoblot analysis of HSP110, Bcl-xL, Bcl2, and cleaved caspase-3 (Casp3) at 48 hours after transfection of HBL1 with an shRNA targeting HSP110 or a control shRNA (n = 3). β-actin served as a loading control. ns, not significant. ***P < .001.