Mutational analysis of DLBCL cell lines and EVs. Mutations from DLBCL cell lines are listed in red circles and those from EVs are listed in blue circles with total number of known mutations from exome-seq (green rectangle) demonstrating that several mutations from cell lines are detected in EVs. RNA sequencing indicated that all mutations found in EVs were also present in the cell of origin, but low coverage caused them to be filtered out in the analysis. This technical issue also explains the small number of mutations shared between OCI-Ly7 cells and corresponding EVs, although this could reflect a difference in biology between cell lines. The mutations shared between cell lines and EVs could ultimately provide a way in which disease status could be monitored via liquid biopsy in DLBCL patients.