Effect of exogenous broad-spectrum metalloproteinase inhibitor on stored platelets. Platelets were stored under blood-banking conditions for 7 days in the absence or presence of 10 μM marimastat (n = 18). (A) Platelet volume changes from day 1 to day 7 and in response to marimastat. Mean platelet volume (n = 18) for platelets stored with 10 µM marimastat (black bars) or DMSO vehicle control (empty bars). *P < .05 (paired t test with Bonferroni correction). Error bars indicate standard deviation of the mean. (B) Marimastat effect on platelet volume (P = 2.2 × 10−5), concentration (P = 1.3 × 10−5), and ESC (P = .03) on day 7 expressed as log2(marimastat/DMSO). Gray line indicates no change (ratio of 1); P values represent results of paired t tests. Arrows point out the parameters of the platelet unit analyzed by proteomics. (C) Linear correlation between platelet volume and ESC on day 7 expressed as log2(marimastat/DMSO). P value and correlation coefficient are shown.
