RNAi screen for regulators of mutp53 accumulation. (A) Screen layout. After lentiviral transfer of a pooled shRNA library, cells were sorted by flow cytometry into p53-low and p53-high cells. shRNA barcodes were amplified and their abundance was determined by high-throughput sequencing. The experiment was performed in technical duplicates. (B) Screen results. Data from multiple shRNAs per gene were combined using the wZ method, which assigns a high score to genes targeted by multiple enriched shRNAs. wZs were calculated for p53-low and p53-high cell populations by comparison against the input samples. Boxes highlight the top and bottom 10 shRNA-targeted candidate genes enriched or depleted in the respective population. SSC, side scatter.