Effect of plerixafor and/or G-CSF administration on number of nucleated cells in peripheral blood and bone marrow. (A) Peripheral blood and bone marrow were harvested from 5 subjects prior to (baseline) and at 4 and 24 hours after administration of plerixafor (240 μg/kg). Total nucleated cells were isolated from peripheral blood and bone marrow. Statistical significance was determined by the 2-tailed paired Student t test. (B) Peripheral blood was harvested from subjects (1) after 4-daily administration of G-CSF (5 μg/kg); (2) after 4 hours of plerixafor (240 μg/kg) administration; and (3) after 4-daily injections of G-CSF followed by plerixafor administration. Total nucleated cells were isolated from peripheral blood. Statistical significance was determined by the 2-tailed Student t test.