Genealogic analysis of large memory B-cell clones with intraclonal diversity. (A-C) Selected lineages of expanded clones in the NCSM B-cell subset. (D-E) Selected lineages of expanded clones in the CS memory B-cell subset. (F) Selected lineage of the second largest clonal expansion discovered in this study in the NCSM B-cell subset. The grayscale indicates whether a given clonal member was found in replicate 1, in replicate 2, or in both (legend). The germline IGHV gene is given as the root of the tree, and any sequential mutation event between the germline and the clonal members is shown as a small black circle. The lineages belong to the following clones: (A) Pt1, NCSM, IGHV4-59_IGHJ4, members (replicate 1/replicate 2): 7/7; (B) Pt5, NCSM, IGHV3-43_IGHJ4, members: 8/7; (C) Pt12, NCSM, IGHV3-30_IGHJ4, members: 7/6; (D) Pt23, CSM, IGHV6-1_IGHJ6, members: 11/7; (E) Pt14, CSM, IGHV4-34_IGHJ5, members: 5/5; (F) Pt31, NCSM, IGHV1-69_IGHJ4, members: 185/137.