Clonal-related B cells in HCV patients and healthy donors in the 4 B-cell subsets. (A) B-cell clones present in both replicates. Clones present in both replicates, shown as points according to their composition with x = clonal members and y = total read count. A higher concentration of larger clonal expansion can be observed in the NCSM B-cell subset of HCV-infected individuals. Indicated is the number of reads per clone member in both replicates. (B) The percent of reads in expanded clones relative to the total number of processed reads is shown for the 2 memory B-cell subsets for all individuals. The expanded clones considered here are the combination of clones with intraclonal diversity and at least 5 members in each replicate (supplemental Table 5) and the clones without substantial intraclonal diversity but an outlying number of reads (supplemental Table 6). The y-axis is shown on a “square-root scale.” In the figure, the average percent of reads in expanded clones per subset and per study group is given, with the 95% CI given in parentheses.