TOX2-KD effects on receptor acquisition, degranulation function, and perforin expression. (A) Control or TOX2-KD–transduced CD34⁺ cells were subjected to in vitro NK cell differentiation. Flow cytometry was performed on day 17 to analyze the expression of various inhibiting and activating surface receptors (NKG2A, NKG2D, NKp46, NKp30, NKp44, and CD16) on the GFP⁺CD56⁺ cell population. Results are representative of 3 independent experiments. (B) CD107a mobilization on K562 stimulation in the GFP⁺CD56⁺ cell population was analyzed by flow cytometry. Results are representative of 3 independent experiments. (C) Expression of perforin was compared between control and TOX2-KD GFP⁺CD56⁺ cells on day 17 by flow cytometry. Results are representative of 3 independent experiments.